Initialising ...
Initialising ...
Initialising ...
Initialising ...
Initialising ...
Initialising ...
Initialising ...
Kado, Masataka; Kishimoto, Maki; Tamotsu, Satoshi*; Yasuda, Keiko*; Aoyama, Masato*; Tone, Shigenobu*; Shinohara, Kunio*
AIP Conference Proceedings 1696, p.020019_1 - 020019_4, 2016/01
Times Cited Count：3 Percentile：85.37(Microscopy)Soft X-ray microscope is a very powerful tool to observe cellular organelles of living biological cells and many works have demonstrated imaging of inner structures of the cells. However the inner structures are very complicated and it is difficult to identify the organelles obtained with the soft X-ray microscopes. We have proposed a hybrid imaging method with a soft X-ray microscope and a fluorescence microscope that is to observe the same biological cells with the both microscopes at the same time. Using the information of the cellular organelles obtained with the fluorescence microscope, inner structures obtained with the soft X-ray microscope are accurately identified. We have observed living biological cells by the hybrid imaging method. Since the soft X-ray microscope has higher spatial resolution than that of the fluorescence microscope, fine structures of the cellular organelles in the living biological cells were discussed.
Kado, Masataka; Kishimoto, Maki; Tamotsu, Satoshi*; Yasuda, Keiko*; Aoyama, Masato*; Shinohara, Kunio*
no journal, ,
Laser-plasma soft X-ray source produced by a high power pulsed laser is extremely bright and very suitable for biological X-ray microscopy to capture an image of living specimens for which require a single flash exposure to avoid imaging any damages on the specimens. We also have invented to use a fluorescent microscope to identify the cellular organelles in the images obtained with the soft X-ray microscope. The biological cells were cultivated directly onto the PMMA photo resists and observed with the soft X-ray microscope and the fluorescent microscope at the same time. The obtained soft X-ray images and fluorescence images of the cells were directly compared and each cellular organelle such as mitochondria, actin filaments, and chromosomes in the soft X-ray images were clearly identified. Since the soft X-ray microscope has higher spatial resolution than that of the fluorescent microscope, fine structures of the cellular organelles in the hydrated biological cells were observed.
Kado, Masataka
no journal, ,
Soft X-ray wavelengths between absorption K-edges of Oxygen and Carbon (2.3 nm and 4.4 nm) are so called "water window" and the X-ray were well absorbed by Carbon and less absorbed by water. Soft X-ray microscope using the water window X-ray as the light source has advantage to be able to observe live biological cells without any artifacts and can observe fine structures of cells compared to the light microscope. Combining with bright and short-pulsed laser-plasma soft X-ray the soft X-ray microscope which named a laser-plasma soft X-ray microscope can observe live biological cells in situ without radiation damages. We have generated bright water window soft X-ray irradiating a high power laser with 1053 nm in wavelength, 20 J in pulse energy and 600 ps in pulse duration onto thin foiled gold targets. Cultivating biological cells directly on the PMMA photoresists in situ observation of live biological cells with the laser-plasma soft X-ray microscope has been realized.
Kado, Masataka; Kishimoto, Maki; Tamotsu, Satoshi*; Yasuda, Keiko*; Aoyama, Masato*; Tone, Shigenobu*; Shinohara, Kunio*
no journal, ,
Laser-plasma soft X-ray sources have advantage of brightness and short pulse duration and a soft X-ray microscope using them can observe live biological cells with a high spatial resolution. We have succeeded to observe fine structures of cellular organelles of live biological cells and also structural deformations of apoptotic nuclei.
